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R&D Systems Hematology goat anti human trail
Goat Anti Human Trail, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+human+trail/Human+TRAIL%2FTNFSF10+Antibody/pm36947620-418-9-12
Average 93 stars, based on 37 article reviews
goat anti human trail - by Bioz Stars, 2026-10
93/100 stars

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Article Title: Breast tumors interfere with endothelial TRAIL at the premetastatic niche to promote cancer cell seeding
Article Snippet: Coverslips were mounted with ProLong Gold Antifade Mountant without DAPI (Invitrogen, P36930).

Article Title: Breast tumors interfere with endothelial TRAIL at the premetastatic niche to promote cancer cell seeding.
Article Snippet: Livers were dissected, mince, and collected in 50-ml Falcon tubes containing 5 ml of liver digestion buffer [DMEM high glucose supplemented with 1% penicillin-streptomycin (Gibco), 1× sodium pyruvate (Gibco), 1× MEM nonessential amino acid solution (Gibco), collagenase I (1 mg/ml; Gibco, 17100017), collagenase II (1 mg/ml; Gibco, 17101015), Dispase (2.5 U/ml; Gibco, 17105041), and DNase I (20 U/ml; Sigma-Aldrich, D4527)] and placed at 37°C for 30 min with regular vortexing to allow dissociation.

Blocking Assay:

Article Title: Breast tumors interfere with endothelial TRAIL at the premetastatic niche to promote cancer cell seeding
Article Snippet: .. The following primary antibodies diluted in blocking buffer were incubated overnight at 4°C: goat anti-human TRAIL (R&D, AF375; 1:25), rabbit anti–Ki-67 (Abcam, ab15580; 1:1000) and mouse anti–NF-κB (Cell Signaling Technology, 6956; 1:400). .. Appropriate secondary antibodies were used: Alexa Fluor 488–, Alexa Fluor 647–, or Alexa Fluor 568–conjugated secondary antibodies (Molecular Probes; 1:200).

Incubation:

Article Title: Breast tumors interfere with endothelial TRAIL at the premetastatic niche to promote cancer cell seeding
Article Snippet: .. The following primary antibodies diluted in blocking buffer were incubated overnight at 4°C: goat anti-human TRAIL (R&D, AF375; 1:25), rabbit anti–Ki-67 (Abcam, ab15580; 1:1000) and mouse anti–NF-κB (Cell Signaling Technology, 6956; 1:400). .. Appropriate secondary antibodies were used: Alexa Fluor 488–, Alexa Fluor 647–, or Alexa Fluor 568–conjugated secondary antibodies (Molecular Probes; 1:200).

Article Title: Breast tumors interfere with endothelial TRAIL at the premetastatic niche to promote cancer cell seeding.
Article Snippet: For the proximity ligation assay (PLA) of TRAIL and DR5, the Duolink In Situ Red Starter Kit Goat/Rabbit (Sigma-Aldrich, DUO92105) was utilized according to the manufacturer’s protocol, which was combined with immunofluorescence of VE-cadherin. .. The following primary antibodies were incubated at 4°C overnight: goat anti-human TRAIL (R&D, AF375; 1:25), rabbit anti-human DR5 (Cell Signaling Technology, 8074; 1:50), and mouse anti–VE-cadherin (BD Biosciences, 610252; 1:100). .. Donkey anti-mouse Alexa Fluor 488 (Molecular Probes; 1:200) was incubated together with Riera-Domingo et al., Sci.



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